10.2.1 Enzymes Involved in DNA ReplicationHappy Scribe's Favorites
- 927 views
- 26 Oct 2020
Before we delve into the details of replication, let's familiarize ourselves with the enzymes involved in DNA replication. There are three proteins involved in the unzipping of the DNA and preparing the template to be replicated. HeLa case, which is shown in this blue watch here, unwinds or unzips the parental double helix. And immediately after a single strand binding proteins will come over to stabilize the DNA, the single strand DNA structure. So these purple spheres are the single strand binding proteins.
A few steps ahead of Helix is Toppo. Sohmer is busy correcting the overwintering of the DNA helix still intact. And this is the sort of greenish circle that surrounds the DNA double helix. And so what it's doing, it's if you think about how Helix case creates a gap between the two DNA strands and pushes the wiring from the center of the gap outwards, you realize that what the DNA at the edge is doing, it's just quailing, tighter and tighter and tighter.
And so top Saumarez cuts the DNA, unwinds the DNA helix, and then attaches the DNA back together to prevent it from over winding. So there are three other main enzymes involved in the creation of a new DNA strand. And these are primate's DNA, polymerase and DNA. Ligeti's and DNA polymerase is also split into one and three and Prima's shown here begins a new strand. This is Prima's, so begins a new strand of DNA by first actually synthesizing an RNA primer or a short RNA sequence that complements the original DNA.
And the reason why it's not DNA is because DNA can't start from scratch. It has to be attached to something, whereas RNA can. So the primers will synthesize the RNA primer and then following DNA polymerase can begin synthesizing DNA from the RNA primer. And so this orange little chunk is the DNA polymerase and DNA minus three is the one that will continue creating the new complementary strand from the polymer DNA polymerase. One has a job of replacing the RNA primer with DNA after the new complementing strand is fully synthesized and both polymerase enzymes also are proofreading the DNA strands as they synthesize them.
A few steps behind polymerase as DNA Ligeti's, which is this blue chunk here and what Linus is doing, it fuses together the sections of DNA strands. So, for example, right after the DNA polymerase replaces the RNA primer, Ligeti's will come over and fuse the new section of DNA to the rest of the newly synthesized strand. So you can see this is a sort of section of DNA that's not that sort of free-floating other than being attached to the complementary strand.
Ligers will come over and bridge this little gap and those are the enzymes for DNA replication.